Archive for the ‘DNA Test’ Category

DNA testing for the purposes of the experiment

According to DNA test results, World War Two Nazi dictator Adolf Hitler is though to have African and Jewish genes inherited from ancestors.
In the DNA tests carried out, results showed that saliva samples taken from thirty nine relatives of Hitler showed he could well have been biologically linked to what he referred to as the “subhuman” races which he attempted to wipe out during the devastating Holocaust which he was at the helm of.
Marc Vermeeren, a historian and Jean-Paul Mulders, a Belgian journalist, traced the Fuhrer’s relatives including an Austrian farmer –Hitler’s cousin, early on in 2010.
Haplogroup E1b1b1 is the chromosome which showed up in their samples – a rarity in Western Europe, but more commonly found amongst the Berbers of Algeria, Tunisia and Morocco, as well as with Sephardic Jews and Ashkenazi.
The Haplogroup E1b1b1 relates to eighteen to twenty per cent of Ashkenazi Jews and eight point six per cent to thirty per cent of Sephardic Y-chromosomes seems to be one of the main founding extractions of the Jewish world population in general.
In Belgian magazine Knack, Mulders wrote that, “One can from this postulate that Hitler was related to people whom he despised”. The magazine, in which the findings of the DNA test were published, stated that the DNA testing for the purposes of the experiment was carried out under strict laboratory regulations. Roddy Decorte, a genetic specialist bases at the Catholic University of Leuven called the results “surprising”, saying that “”The affair is fascinating if one compares it with the conception of the world of the Nazis, in which race and blood was central.”
According to the results of the DNA tests, Hitler was not, as he perceived himself to be, “Aryan” or “pure”, a term which was used by the Nazi party to suggest generally Caucasian, fair haired and blue eyed people. People suspected to be homosexual, Jewish or black amongst numerous other social, cultural religious and racial groups were ostracized from society according to Nazi law, resulting in the terrible atrocities of the Holocaust.
However, the results of the DNA test are not the first time it has been suggested that Hitler has Jewish ancestry. It is thought that Hitler’s father Alois, is the illegitimate offspring of a maid, Maria Schickelgruber and a young Jewish man, Frankenberger.
The process used to carry out the DNA tests is known as Genealogy, which uses samples such as buccal swabs (large cotton bud-like instruments used for gathering DNA samples in the form of cheek cells from the inside of the mouth) This form of DNA testing has become popular in recent years as people use it to determine their ancestral lineage. Other forms of DNA testing are also used to determine more immediate relations, such as paternity testing.

The results of the DNA tests

According to US researchers, a basic DNA test taken at Stanford University could be used to predict the most effective food plan for a person on a diet, revealing whether a low carbohydrate or low fat diet will produce the most successful weight loss results.
Results taken from a small primary study of 101Caucasian women showed that the women who were on the diet that was considered the best for their individual genetic make up lost 2-3 times more weight than the other subjects.
The results of the DNA tests, which were presented at an American Heart Association conference, were considered inconclusive by experts, who stated that further investigation should be taken.
Nutrigenomics and nutrigenetics is an emerging aspect of DNA test which looks into the interaction of different foods with various gene types.
Historically, people have been aware for sometime that different humans have different reactions to certain foods depending on their genetic makeup, for example lactose intolerance, which is more common among African and Asian people than among Northern Europeans. In DNA testing, dietary treatments based on the results have been implemented in the reduction of disease risk in a number of cases. Past nutritional research placed a lot of emphasis on how deficiencies in various nutrients affected health. Now with nutrigenomics, more emphasis is placed on the optimisation of personal diet to optimise nutritional benefit and to help prevent illness.
The DNA testing explored how efficiently people with different genes managed to lose weight on a variety of weight loss diets.
The DNA test was taken using buccal or cheek swabs taken from the 101 women, who all undertook different diets over the course of a year. The diets were either low fat, very low fat, low in protein or carbohydrate or simply low carbohydrate. Those who were on a diet which was a good match with their genetic makeup found that they lost 2-3 times more weight over the 12 month period in comparison with those who were on an “incompatible” diet.
The group of women were divided into three genotypes. The first was a balanced diet responsive genotype, the second was a low carbohydrate diet responsive, and the third was a low fat diet responsive.
The researchers responded to the DNA tests by saying that the results were only introductory, and much more study would need to be carried out before the results could be used on a commercial basis. Professor Christine Williams of the University of Reading said of the DNA testing carried out in the study: “This is a very intriguing study –though very small.”, going on to suggest that more conclusive results could be reached if researchers could gain a better understanding of the genes involved. However, she also stated that the DNA test correlated with much of the research carried out at Reading, where it was discovered that some subjects responded better to certain fats such as omega 3.

The process of carrying out a DNA test

Deoxyribonucleic acid, other wise known as DNA, forms the genetic blueprint for every living organism on the planet. In a sense, one human being is indistinguishable from another in terms of DNA similarity; however there are a few minor exceptions which mean that some humans are more similar to others as they are connected as families or “blood relations”. To explain: 99.9% of human DNA sequences are the same in each individual, however there is enough DNA to be able to differentiate one person from another. In DNA testing, repeat or repetitive sequences are used which are highly variable, known as variable number tandem repeats or VNTR –loci which are highly similar between two persons related to one another –so similar that unrelated humans are very unlikely to have the same variable number tandem repeats as the relations.
It is this form of DNA which is often used for DNA testing, which has a number of different applications. The process of carrying out a DNA testor DNA profiling as it is also known was first described by Sir Alec Jeffreys for the University of Leicester. It now forms the basis for many national DNA databases. Jeffreys also pioneered the genetic fingerprinting technique, which was made commercially available at chemical company ICI in 1987 when the company opened a centre for blood testing.
Genealogy is a common form of DNA test which has recently been popularised by the current trend for tracing a person’s origins through birth, death and marriage records online. However, this method of tracing one’s ancestry can be fairly limited due to records running out or missing altogether, and as a result many are turning to DNA tests in order to trace their genetic lineage.
DNA testing is also used to determine more recent family ties, for example in paternity and maternity testing. This form of DNA test normally involves a buccal swab taken of each subject prior to being taken to the lab to be compared and contrasted. These DNA tests are also used for other family methods and also for twin or zygosity testing.
Samples for DNA tests can be taken from anywhere on the human body. Common samples which are used include blood samples, saliva, skin cells, hair and sperm samples. These are often collected at crime scenes to provide legal evidence, as are fingerprints, which are directly related to the individual makeup of a person’s DNA. There has been some speculation as to whether a criminal would be able to plant “fake” DNA at the scene of a crime (Which has previously been attempted), but according to scientists, in order for this to be able to cover a criminal’s tracks sufficiently, they would need a wealth of laboratory equipment and a lab technician or engineer on hand.

A DNA test can also be used for legal purposes

The process used by forensic scientists to assist in the identification of individuals based on their individual DNA profiles. A DNA profile is an encrypted set of numbers which is used in reference to the person’s individual genetic make up, which can then be used to identify a person. This is referred to with several terms: DNA testing, profiling, typing or genetic fingerprinting. DNA tests are often confused with full genome sequencing, which is where the total DNA sequence of the genome belonging to an organism is analysed at one time. A DNA test may be taken in instances where a person wishes to establish biological links between themselves and another, for medical research or to provide criminal evidence.
A DNA test may be carried out for a number of different purposes –to determine ancestral lineage for example, in genealogy, to determine biological links within a family, or to be used as evidence in legal procedures. DNA tests are even carried out for scientific purposes in order to carry out research into life threatening illnesses and of course to gain further insight into the matter of genetic testing itself.
Remarkably, ninety-nine point nine per cent of DNA sequences are a match for every human being, although in each person there is enough information there to be able to distinguish one person from another. In DNA tests, repeat sequences are used which change regularly, called VNTR or tandem repeats. The loci of these tandem repeats are highly similar to the loci of those who are closely related to each other, whereas unrelated persons are unlikely to hold the same VNTRS, as the loci are particularly mutable.
Sir Alec Jeffreys, founder of the genetic fingerprinting technique, also founded the DNA testing technique at the University of Leicester in 1984. This pioneering method is now used as the foundation of several national DNA databases.
DNA testing is usually carried out by taking a sample for testing such as a cheek or buccal swab from the person being tested. In doing this, a sufficient amount of cheek cells are gathered to be taken to the laboratory to be analysed. Other than buccal swabs, other samples can also be taken, from saliva, semen, blood, or any other bodily tissue or fluid which can be obtained from razors or combs, for example.
A DNA test can also be used for legal purposes, however it is not unheard of for criminals to plant “fake” DNA samples at the scene of the crime in order to deter investigators. Duplicitous behavior notwithstanding, British experts say that this particular technique of attempting to cover one’s tracks, although possible scientifically, is in fact not likely to be carried out by criminals because the process would require expensive equipment, an experienced biologist or lab technician and a full molecular laboratory.

This is not to imply that such testing needs to be banned outright

Deoxyribonucleic acid is the “building blocks” which make up the basis for every single living organism on Earth. In recent years, DNA or rather, the process of DNA testing has become a source of contention for many. DNA tests can now be purchased inexpensively and carried out quickly, bought on the internet for a relatively small amount of money. But the results provide more than just genetic evidence. In some cases, a veritable Pandora’s Box of controversy is unleashed, and it is this which has been at the centre of much discussion concerning DNA testing.
A DNA test is taken using a procedure which is referred to as buccal swabbing. Two swabs are taken from the left cheek, and two from the right, from the person undergoing testing. These swabs are then run to the lab for analysis, and should certain matches be found in the evidence taken, then some conclusion may be drawn. Generally, this form of DNA test is carried out to determine parentage, although close relative testing is also used to determine other familial links such as grand parentage, tests to check whether a person is an aunt or uncle, or Twin or zygosity DNA testing.
Other reasons for carrying out a DNA test include genealogical testing, which is the process of tracing ancestry using genetic material. Testing is also carried out for the purposes of medical research, and DNA tests are also carried out at the scene of a crime, or to provide evidence in a court case.
In terms of testing for the purposes of determining parentage or other familial links, there are many who view “blood” parentage as a crucial issue –particularly when people who are adopted are taken into account. This then widens the field for debate even further –there are now a significant number of adoptees seeking out their parents or other family ties through testing, driven by a strong urge to discover more about their past, and themselves. Likewise, the subject of blood ties appears to become murkier still when the subject of the male psyche is taken into account. Many of the websites offering DNA tests to fathers who suspect that their children may not be biologically “theirs”, appears to target a sense of mistrust. Regardless of this, the NHS website for such testing emphasises that a test be carried out “in the child’s interest”, suggesting that when ownership is weighed up against the process of raising and caring for a child, it can be a hard issue to deal with –but ultimately parenting wins. 2005 saw on average 10,000 paternity tests taken in a single year, many of which were checked by the CSA or Child Support Agency. Despite this, there is still a sizable amount which go unchecked –tests which can be carried out at home, unsupervised, with no guarantee of accuracy –leaving those involved to deal with the consequences.
This is not to imply that such testing needs to be banned outright. The grey area presented by the issue, and the accompanying heartache –or for some, celebration- that comes with it suggests that there could be better controls placed on the process, and a clearer understanding of the issues involved.

Genealogical DNA tests are a way of providing conclusive evidence

Recent years have seen a burgeoning trend towards genealogical DNA testing as a means of gaining a better understanding of ourselves, as well as adding those extra branches to a family tree which ordinarily would have ran out due to absent, incomplete or lost records.
The success of television programmes such as Who Do You Think You Are? is perhaps telling of a generation of people now more in search of identity than ever before, and looking to the past for clues and answers as to how, why and most importantly who we are. Some speculate that the desire for DNA testing to be carried out goes beyond the personal into the wider community. Especially in urban areas, themes of identity can become fraught as people look to the past in order to make sense of the present, and along with modern advancements in science, people are now looking to genealogical DNA tests to provide them with some clues.
Genealogical DNA testing is commonly misconceived as being the same as the process used to analyse DNA at crime scenes or for medical reasons. This is in fact untrue –the way in which DNA tests are carried out in the field of genealogy is far more restricted, so that rather than showing details of hereditary illnesses and so forth, it uncovers links to people in history –distant relations and people from a long-forgotten past. In a sense then, a genealogical DNA test deals mainly with the business of genealogy –tracing family lineage.
Genealogical DNA tests are a way of providing conclusive evidence regarding the origins of our families, going back even as far as tens of thousands of years. Among non-Europeans, twenty-nine clans have been discovered. Four belong to Native Americans, twelve belong to people of African origin, and nine belong to those with Japanese heritage. Interestingly, around half of Europeans are thought to be descended from one woman Helena, who lived around 20,000 years ago in the Pyrenees. Helena is one of seven women, or “the seven daughters of Eve”, who are thought to be the original women from which most people of European extraction are descended.
It is now possible to take a DNA test which can open up a whole range of links to a person’s past, simply by using a cheek swab. The evidence found on the swab is a product of a mitochondrial DNA test or mt DNA, genetic material which is carried from mother to child and therefore does not tend to alter significantly. In spite of this, gaps can still be produced as a result of this form of testing as it only accounts for two lineages –the mother and father, both ten times removed, not allowing for any additional information regarding others who would have added to the person’s overall genetic “picture”.

DNA testing works by taking a reference sample

Genetic fingerprinting, also known as DNA typing, DNA testing or DNA profiling, is the process used by forensic scientists to help identify individuals based on their individual DNA profiles. A DNA profile is the encrypted set of numbers which refers to an individual’s genetic make-up. This can then be used to identify a person. DNA tests are commonly confused with full genome sequencing, which is a process used to determine the total DNA sequence of the genome of an organism at one time. A DNA test, however, is often taken to determine biological familial links and to provide evidence for crimes.
99.9% of human DNA sequences are a match in every person, however there is sufficient DNA available to be able to tell one individual from another. DNA tests use repeat sequences which vary highly, known as variable number tandem repeats, or VNTR. The loci of VNTRs are very much alike between persons who are closely related to one another, but unrelated humans are not likely to have the same VNTRs, as the loci are highly variable.
Sir Alec Jeffreys was the founder of the DNA testing technique at the University of Leicester in 1984. This technique now forms the basis for several national DNA databases. Dr. Jeffrey also pioneered the genetic fingerprinting technique, which became commercially available in 1987 when chemical company ICI opened a centre for blood testing.
DNA testing works by taking a reference sample, usually a buccal or cheek swab from an individual. This removes enough cheek cells from the person to be used for lab analysis. Other samples can also be used for the purposes of a DNA test such as blood, semen, saliva or any other tissue or fluid obtained from items such as hairbrushes or razors.
In some cases, where DNA testing has been used for the purposes of solving crimes, criminals have been known to plant fake DNA samples by fabricating DNA such as hair or blood and planting it at the crime scene. However, British experts have stated that this method of covering up a crime, while scientifically possible, is unlikely to be carried out by criminals, as it would require expensive equipment, a full molecular laboratory and an experienced lab technician or biology scientist.
A DNA test can also be used to determine relations between one person and another, for instance to determine the biological parent of a child, and some DNA tests can trace family lineage as far back as thousands of years ago. This form of genealogical DNA testing can produce a variety of interesting findings for individuals which allows them to build up a more complex family tree than would be possible by looking at records alone.

Using DNA testing to trace their ancestry

In recent years, particularly with the rise in television programmes such as Who Do You Think You Are?, the matter of tracing one’s ancestry has become increasingly popular, and more and more people are looking to the past for clues as to their origins.
One common myth is that genealogical DNA testing, the process by which a DNA test is carried out to determine ancestry is the same as DNA tests which are carried out for medical or criminal purposes. This is untrue –the process used to carry out the DNA tests is more restricted, and instead of revealing details such as hereditary illness and so on, uncovers links to the past, even people who we may be distant relatives of, without even being aware of it.
There is some speculation that this could be something to do with the current society we live in. As a group of people, particularly in urban areas, many feel a sense of disconnection from others, and a sense of community and family having been lost. Now amateur genealogists are going further than most family trees or records will be able to, using DNA testing to trace their ancestry.
Most records of births, deaths and marriages will only be able to go so far before the evidence runs out due to absent, incomplete or lost records, or even the records having not begun officially. DNA testing is a way of providing conclusive evidence tracing the origins of our families as far back as tens of thousands of years.
Among non Europeans, twenty nine clans have been found, including twelve belonging to African origin, four among Native Americans, and nine among Japanese. About half of Europeans are thought to be descendants of Helena, a woman who lived in the Pyrenees 20,000 years ago, and most Europeans are descended from one of seven women, or “the seven daughters of Eve”, as they have been dubbed.
Now it is possible to take a DNA test which will determine the ancestry of a person by taking a cheek swab, running it to the lab for analysis, and tapping into a vast database. The evidence which is produced from mitochondrial DNA tests or mtDNA, tests genetic material which is passed down from mother to child and alters little. However this form of DNA testing can still produce gaps as it only takes into account two lineages; the father ten times removed, and the mother ten times removed, and so does not allow for any information about others who would have contributed to a person’s genetic makeup.
Genealogical DNA testing is not only a good way of building up a rich and fascinating history of a family, but it is also a method of tracing the migratory patterns of these ancestors, imagining their journey, and a key to understanding our present selves.

Why DNA test to be carried out.

DNA, otherwise known as deoxyribonucleic acid, otherwise known on numerous science television programs as “the building blocks” which form the basis for every living organism on the planet, is a tricky business, particularly when it comes to DNA testing.
Once the stuff of science fiction, now inexpensive and quick DNA tests can be purchased on the internet for a small fee, and you can’t turn on the TV mid-morning without someone on a chat show demanding to know who the father of his child is. Of course, the availability of DNA testing has proved to be something of a minefield, sparking widespread controversy, protest and in many cases, upset to the family involved.
A DNA test is carried out using a process known as buccal swabbing, where four swabs are taken (Two from the inside of each cheek) from each person. The results are then sent to laboratory, and if certain matches are found in the DNA, then a problem can be resolved …or created. As well as the paternal or maternal testing, close relative DNA testing is available for tests to determine whether or not a person is grandparent, uncle, aunt, niece or nephew, siblingship testing and Twin or zygosity DNA testing, amongst many others.
There are many important reasons for a DNA test to be carried out. Some DNA tests are carried out for ancestry reasons, to determine a person’s historical origins. Others are carried out to test for the likelihood of illnesses such as cancers, and DNA tests are also carried out for legal reasons, for example to provide evidence in court.
In 2005, an estimated 10,000 paternity tests were carried out each year. And while many of the checks for these tests were carried out by the CSA or Child Support Agency, there are also a number of internet DNA testing services available which can be carried out at home.
For many, “blood” parentage is still an important matter, linked to old traditions concerning identity and place in society, and the issue becomes even more complex when it is linked to the male psyche, with many internet DNA testing websites even appearing to target a sense of paranoia many fathers may be experiencing about the paternity of their child, with the feeling that, if it is not genetically “theirs”, it is not theirs, full stop. And whatever issues arise from the results of a DNA test, DNA testing is not the measure of how well a child is loved or cared for. Indeed, the NHS website for paternity and maternity testing insists that if a DNA test is to be carried out, it needs to be, “in the child’s interest”.
This is not to suggest that DNA testing should be banned. It is not a clear cut problem, and therefore cannot have an equally defined solution. Whether adopted people, for instance, should be denied the opportunity to discover their biological parentage is just one of the questions being thrown into the already murky quandary concerning DNA tests, suggesting that perhaps tighter controls need to be made over DNA testing, and counselling offered to families who may not yet understand the implications of the results.